Fig. 3. Activation of KCNQ1/KCNE1 by DIDS is associated with a reduced rate of channel deactivation. A,B. Examples of KCNQ1/KCNE1 deactivating current traces before and after application of 10 µM DIDS at two different KCNQ1/KCNE1 ratios. Currents were recorded using the deactivation protocol described in methods section. C,D. DIDS increases the time constant of deactivation determined by fitting traces to a single exponential function (Q1/E1 1:0.67 n = 13-23; Q1/E1 1:2 n = 7-11, ± SEM).